JF Fortessa HTS Laser Chart. M Canto Laser Chart JF Fortessa Laser Chart. Protocols. Cell Surface Staining Protocol. BD HTS User Guide

BD High Throughput Sampler User’s Guide For the BD LSRFortessa I. Introduction The BD High Throughput Sampler (HTS) is a high-speed sampling device used on the 5-Laser LSRFortessa located in Rm. 6006F of the RIA Division. The HTS provides users the ability to acquire samples from 96-well or 384-well plates for efficient analysis with

This  LSR Fortessa cell analyzer (BD Biosciences) uses up to five lasers (355 nm, 405 BD™ High Throughput Sampler (HTS) allows sample loading from 96-well  29 May 2014 BD FACS LSR Fortessa User Manual. Flow Cytometry Unit – Biomedical Core facility. Technion Faculty of Medicine. For help please call:. Throughput Sampler (HTS) option for the BD FACSCalibur™ multiple times with user-defined mixing, wash, and analysis Training.

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M Canto Laser Chart JF Fortessa Laser Chart. Protocols. Cell Surface Staining Protocol. Intracellular Staining Protocol Whole Blood Lysis Protocol. Ultracomp Bead Staining Protocol. FlowMetric Whitepapers.

Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X-20 cell analyzer gives you the power to expand and customize your science. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential.

Flow Cytometry Unit – Biomedical Core facility. Technion Faculty of Medicine. For help please call:.

Instrument Guides. Work in progress. Quick Guides. Fortessa/LSRII; Attune NxT; BD FACS Aria BioLegend · BD Biosciences BD Accuri C6 BD LSR-II HTS.

This allows researchers to optimize assay design by selecting the latest fluorescent dyes and antibodies in experimental protocols. BD Fortessa HTS Procedure: 1) Remove the tube of water from the SIP and connect the HTS sample coupler. Leave the support arm to the side.

Each The Danish Stem Cell Center (DanStem) Appendix’B 448 nm (standard) 488nm (GFP/YFP discrimination) A B C A B C 685LP, 710/50 505LP, 530/30 488/10 525LP, 542/27 505LP, 510/20 488/10 Official manuals from BD for the following: 1) LSRII 2) Fortessa/X-20 3) HTS for users 4) HTS for service. FACSAria Guides. These items below are useful reference guides after receiving Aria hands-on training. Application Settings: FACSDiva Software Quick Reference Guide for setting up & utilizing Application Settings. The BD LSR Fortessa™ X-20 cell analyzer delivers high-performance multicolor analysis detecting up to 18 parameters simultaneously with using 5 lasers: UV (355 nm) Violet (405 nm) Blue (488 nm) Y-G (561 nm) Red (640 nm) Fortessa X20 Flow Cytometer Quick guide Operation of the 5 laser Fortessa X20 BD Webinar CS&T System BD Compensation Guide… BD LSR Fortessa X20 – How to Use Guide 4 3 At the top of the Browser panel, create a New Experiment (click the icon with a book).
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With up to four different laser configurations and 14 parameters, the cytometer is fully optimized to work with BD Horizon Brilliant ™ dyes, so you can focus on what matters most—your research. SOP for HTS Getting started Switch the cytometer and the computer on. The HTS initialises when cytometer is switched on. Login to DIVA software. Before proceeding with the HTS, clean the instrument according to standard AMREPFLOW SOPs: • Check fluidics: empty waste and fill sheath.

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The BD™ High Throughput Sampler (HTS) option is available to increase your lab productivity by acquiring samples from a 96- or 384-well microtiter plate. The HTS can be front or side mounted on the BD LSRFortessa X-20. HTS Throughput Acquisition: Less than 15 minutes per microtiter plate in high-throughput mode using a 2-second

BD High Throughput Sampler User’s Guide For the BD LSRFortessa I. Introduction The BD High Throughput Sampler (HTS) is a high-speed sampling device used on the 5-Laser LSRFortessa located in Rm. 6006F of the RIA Division. The HTS provides users the ability to acquire samples from 96-well or 384-well plates for efficient analysis with This guide describes how to set up and operate the BD™ High Throughput Sampler (HTS) option with the BD™ LSR II, BD FACSCanto™, and BD FACSCanto™ II flow cytometers.

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Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. 10 BD LSRFortessa Cell Analyzer User’s Guide Instrument overview The BD LSRFortessa is an air-cooled multi-laser benchtop flow cytometer with the ability to acquire parameters for a large number of colors.

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